Regarding the tumor growth change following CAR-T-cell treatment, the intra-tumoral gene expression of two human markers (interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα)), which represent CAR-T cells’ functionality, was analyzed at EDD18 by RT-qPCR, to interpret the action of CD19 CAR-T (CD3z-CD28) cells in ovo. Here, CD28 is linked to neoplasm.