IL6 and neoplasm: Western blot analysis further revealed that LIMA1 knockdown reversed the regulatory effects of LRP5-overexpressing osteocyte-derived CM on tumor-associated proteins: the metastasis-related markers Snail, MMP9, IL-6, and TGF-β1 were upregulated, whereas the expression of the apoptosis-related protein cleaved caspase-3 was reduced (Figure 1K).