Binary logistic regression analysis was performed to assess the association between the promoter methylation status of four investigated genes and GBM disease, as indicated by the odds ratios (ORs) and corresponding (CI) univariate logistic regression analysis, which demonstrated significant associations between promoter methylation of NDRG2 and GLI1 and the development of GBM. This evidence concerns the gene NDRG2 and glioblastoma.