The promoter methylation level of GLI1 was significantly lower in patients with a tumor size of less than 5 cm than in those with a tumor size greater than or equal to 5 cm (p = 0.005), as shown in Figure 2G. There was no significant difference in the methylation of any gene according to the age, gender, or tumor site of the patients, except for GLI1, which showed a significantly higher promoter methylation in patients with multiple tumor sites than in those with a single tumor site (p = 0.045), as shown in Figure 2H. This evidence concerns the gene GLI1 and neoplasm.