Figure 6 displays NLRP3 (~75 kDa) and β-actin (~41 kDa) bands in samples from women with PCOS, while Figure 7 presents corresponding bands from non-PCOS participants. A strong NLRP3 signal is evident in several non-PCOS samples, particularly in VAT. The THP-1 cell lysate used as a positive control confirms antibody specificity with a clear NLRP3 band at ~75 kDa. β-actin served as a loading control in both blots to ensure equal protein loading. The original uncropped Western blot membranes for both Figure 6 and Figure 7 are available in Supplementary Information. This evidence concerns the gene ACTB and polycystic ovary syndrome.