There was no correlation between <i>FLT3</i>-ITD mutation and expression levels of CD117, CD38 or HLA-DR (all <i>p</i> > 0.05).<h4>Conclusions</h4>AML with <i>KMT2A</i>-PTD shows distinctive immunophenotypic features with increased CD123 and aberrant CD25 expression, both associated with <i>FLT3</i>-ITD. Here, CD38 is linked to acute myeloid leukemia.