Whether its protective effect on skeletal muscle in T2DM involves the regulatory control of BDH1 expression remains unclear.<h4>Methods</h4>Wild-type (WT) and systemic BDH1 knockout (BDH1<sup>-/-</sup>) male C57BL/6J mice were used to establish the sedentary control (SED) and AE models of T2DM by providing a high-fat diet combined with streptozotocin injection. Here, BDH1 is linked to type 2 diabetes mellitus.