Other biases might be related to the potential assay variability for cortisol and ACTH (Roche ECLIA), the influence of obesity/overweight (as shown by mean BMI), which independently affects cortisol dynamics, and the fact that overall we analyzed a population with a relatively low prevalence of fractures (4.76%), which might limit fracture-specific conclusions. This evidence concerns the gene POMC and obesity due to melanocortin 4 receptor deficiency.