Existing studies have demonstrated that inhibiting RUBCN expression arrests the cell cycle and suppresses tumor cell proliferation; however, its specific mechanism in breast cancer remains unclear [27], We demonstrated that RUBCN is significantly overexpressed in both cell lines and breast cancer tissues, with its elevated expression being strongly associated with poor prognosis as evidenced by TCGA and GEO datasets. The gene discussed is RUBCN; the disease is breast cancer.