The cTnI_nanoMIP-SPR showed an LOD of 3.53 × 10<sup>-15</sup> M, with a linearity range of 1 pM-100 pM, outperforming previously reported sensor platforms and making it a promising tool for early-stage myocardial infarction detection. Here, TNNI3 is linked to myocardial infarction.