Our study found that berberine treatment can increase TRIB1 expression and improve leptin resistance, thereby adjusting the ratio of OPA1 fusion-promoting form (long, about 100 KDa) and non-fusion-promoting form (short, about 85 KDa), restoring the unbalanced mitochondrial dynamics in obesity, thereby improving the overall quality and function of mitochondria. The gene discussed is TRIB1; the disease is obesity disorder.