Proteins involved in pathways characteristics of aging, such as the immune response (transcriptional regulation by STAT), cancer (transcriptional regulation by SMAD, transcriptional regulation by myc), longevity (transcriptional regulation by FOXO, Sirtuin signaling pathway), and stress responses (HSP90 signaling pathway, MAPK signaling pathway; Zia et al., 2021; Haigis and Yankner, 2010), were enriched in the proteome profiles of milton knockdown flies compared with those of control flies at 7-day-old (Table 2), suggesting that depletion of axonal mitochondria accelerates aging in the brain. This evidence concerns the gene MYC and cancer.