To identify key genes with the most significant impact on CRC prognosis, a univariate Cox regression model was applied to the 180 commonly upregulated genes, revealing 13 genes (ACSL6, AUNIP, CXCL3, CXCL8, CXCL11, EPHX4, GPR143, LRRC8, MAD2L1, MCM2, MELK, MMP3, and NFE2L3) associated with overall survival (OS), all of which acted as protective factors (Figure 4A). This evidence concerns the gene MMP3 and colorectal carcinoma.