To investigate the effects of matrix stiffness on lung cancer cell migration and the Piezo1 channel expression, we prepared PA gels with stiffness close to that of reported healthy (3 kPa, soft) or cancerous (10 and 20 kPa, stiff) lung tissues and cultured A549 or H460 cells on them (Fig. S1). Here, PIEZO1 is linked to lung carcinoma.