Although dysregulation of Sphingolipids and Glycerophospholipids in blood and brain has been previously reported by us and others,61,125-129 ours is one of the first studies to comprehensively investigate changes in brain lipid levels in AD donors carrying rare TREM2 risk variants and the first to use two AD-affected brain regions. The gene discussed is TREM2; the disease is Alzheimer disease.