In addition, cross‐reactivity between scrub typhus and leptospirosis has been attributed to similar mechanisms, including polyclonal activation and non‐specific IgM binding, and therefore misclassification is common unless confirmatory reference tests such as indirect immunofluorescence assay (IFA) or microscopic agglutination test (MAT) are performed [40]. This evidence concerns the gene CD40LG and leptospirosis.