Western blot analysis of Nrf2-related molecular expressions disclosed a notable reduction in the nuclear translocation of Nrf2, along with decreases in the expressions of HO-1 and NQO-1, which are downstream effectors of Nrf2, in the myocardial tissue of mice in the TAC group (Figures 4E–I). Here, HMOX1 is linked to persistent truncus arteriosus.