To investigate the mechanisms underlying POFUT2-mediated angiogenesis in colorectal cancer, we transfected HCT8 cells with a 3xFLAG-POFUT2 plasmid and employed a FLAG-tag antibody to immunoprecipitate POFUT2-interacting proteins for subsequent mass spectrometry analysis (Figure S4B). Here, POFUT2 is linked to colorectal cancer.