The inclusion criteria were as follows: (i) aged 40-75 years; (ii) confirmed CRC diagnosis by colonoscopy and pathological examination; (iii) body mass index (BMI) 18.5-30 kg/m2; and (iv) the absence of distant metastases or curative resection; (V) All of the patients whom were previously selected expressed the 4 major MMR proteins (MLH1, MSH2, MSH6, and PMS2), only patients with proficient MMR (pMMR) status were included, while those with MMR deficiency (dMMR) were excluded. This evidence concerns the gene MRC1 and colorectal carcinoma.