In addition, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) enrichment analysis showed that apoptosis was significantly increased after ischemic brain injury, while p53 signaling pathway, TNF-a signaling pathway and mitogen-activated protein kinase (MAPK) signaling pathway may play an important role in ischemic stroke. This evidence concerns the gene TP53 and ischemic stroke.