SYNGR3 and Alzheimer disease: Studying individual synaptosomes and co-localising the AT8 + tau signal with T181-positive tau at the single-aggregate level, as well as determining the presence of exposed phosphatidylserine (“eat me” signal), CD47 (“don’t eat me” signal), and synaptogyrin-3 in synaptosomes containing AT8 + tau (see Supplemental Figs. 7 and 8 for representative images), we were able to explore how synaptic tau aggregation alters synaptic function and pruning, which are potentially contributing to cognitive deficits in AD.