For the 127 MP cases identified by tNGS, the concordance rates of traditional methods were as follows: IgM serology was positive in 75 of 124 tested cases (60.48%), PCR was positive in 58 of 82 cases (70.73%), and the combination of both methods identified 67 of 82 cases (81.71%) (Figure 4). Here, CD40LG is linked to hyperplastic polyp.