Herein, although the proportion of CD4+ T and CD8+ T cells, as well as PD-1/Tim-3/Lag-3 highly expressed CD4+ T and CD8+ T cells in tumor tissues was minorly changed by sh-TBX21, TNFα/GZMB highly expressed CD8+ T cells were markedly enriched by silencing TBX21, along with remarkably enhanced STAT1/TNFα/GZMB/Perforin levels and reduced anti-inflammatory cytokine levels in tumor tissues. The gene discussed is CD8A; the disease is neoplasm.