Among the 11 candidate biomarkers, seven genes (i.e., IFI44, IFI35, IFI27, IFIT3, OAS3, TNFSF10, and EPSTI1) with no previous reports of association with ZIKV infection were selected for functional verification via siRNA-mediated gene knockdown. This evidence concerns the gene EPSTI1 and Zika virus infectious disease.