Although thyroid peroxidase (TPO) did not display prominent significant associations in the current dataset, given its well – established role as a key enzyme in thyroid hormone synthesis and its previously reported significant positive association (e.g., TWAS.Z = 6.06, FDR = 1.35 × 10 − 9), it remains a strong candidate as an important genetic factor in the pathogenesis of hypothyroidism. The gene discussed is TG; the disease is hypothyroidism.