However, it is diagnosed when at least two of the following clinical criteria are met: multiple café-au-lait macules (CALMs), neurofibromas, characteristic freckles (Crowe’s sign), Lisch nodules in the eyes, specific bone abnormalities, optic glioma, or a first-degree relative with a confirmed NF1 diagnosis (Neurofibromatosis. Here, NF1 is linked to neurofibromatosis.