Quantitative PCR analysis evaluated the mRNA expression of key kidney marker genes, including Tnf, Tlr4, Igfbp7, Kl, Clu, Timp1, Vcam1, Timp2, Apoe, and Cystatin C, in the control, adenine‐induced CKD, and treatment groups. The gene discussed is TLR4; the disease is chronic kidney disease.