Quantitative PCR analysis evaluated the mRNA expression of key kidney marker genes, including Tnf, Tlr4, Igfbp7, Kl, Clu, Timp1, Vcam1, Timp2, Apoe, and Cystatin C, in the control, adenine‐induced CKD, and treatment groups. The gene discussed is IGFBP7; the disease is chronic kidney disease.