Figure 5G–J shows representative confocal images of fixed specimens labeled with Sox2 (magenta) and acetylated alpha‐tubulin (cyan) antibodies, which trace the hybrid‐origin mechanism of SO superficial line development through a series of steps: (1) At 5 mm SL the naked nAVsos nerve extends (white arrowhead) to meet the tissue “bridge” made by the interneuromast cells of AD‐derived neuromasts IO4 and O2 (Figure 5G, brackets). Here, SOX2 is linked to Alzheimer disease.