Hydropic degeneration, focal inflammatory infiltrates, and hepatocyte necrosis were observed in the AP and Alcohol+AP groups.<h4>Conclusions</h4>Multiple AP led to elevated TLR4, NF-κB, IL-6, and TNF-α levels and significant hepatic alterations including hepatocyte degeneration and necrosis. This evidence concerns the gene TLR4 and alkaline phosphatase measurement.