In this study, we investigated the localization and clinical significance of TIM4 in breast cancer.<h4>Methods</h4>We immunolocalized TIM4 in human breast carcinoma tissues using immunohistochemistry (IHC) and multiplex fluorescence-immunohistochemistry (F-IHC) and examined its correlation with clinicopathological parameters and clinical outcomes.<h4>Results</h4>TIM4 was highly expressed in both carcinoma cells and stromal cells in human breast carcinoma tissues. The gene discussed is TIMD4; the disease is breast carcinoma.