We then proceeded to use prepared plasmids to generate lentiviral vector and transduce K562 cells, previously engineered to express a HLA-A*01:01/GZMB-FRET reporter gene cassette (referred to as KFRET.A0101 cell line) (17), at a multiplicity of infection (MOI) of 7. The gene discussed is HLA-A; the disease is infection.