Concurrently, RT-qPCR analysis revealed that the expression levels of tumor proliferation markers (MCM2 and Ki-67) and epithelial-mesenchymal transition (EMT)-related markers (CDH2, VIM, and FN1) were also significantly elevated following TRPM6 knockdown (Figure 8D), consistent with the phenotype assay results. The gene discussed is VIM; the disease is neoplasm.