We compared wild-type UBQLN2 (UBQLN2WT) to three ALS-associated UBQLN2 alleles: a common clinical point mutation (UBQLN2P497H), a 4XALS mutant carrying four clinical mutations (UBQLN24XALS: P497H, P506T, P509S, P525S) previously shown to exhibit increased aggregation and toxicity (Kim et al., 2018; Kim et al., 2023), and a truncation mutant (UBQLN2I498X) that is undetectable by Western blotting due to its instability (Kim et al., 2018; Kim et al., 2023). The gene discussed is UBQLN2; the disease is amyotrophic lateral sclerosis.