CALR and neoplasm: Its immune effect is significant: after ICD occurs, tumor cells release damage-associated molecular patterns (DAMPs), including exposed calreticulin (CRT) and secreted high mobility group box 1 (HMGB1), among which the green fluorescent signal of CRT is significantly enhanced, and the level of HMGB1 reaches 29 pg/mL (1.5 times that of other groups).