Human bronchial epithelial basal cells (HBECs) gradually lose CFTR function over time (Gentzsch et al., 2017) Modifying the standard CRC protocol allowed for the long‐term growth of both normal and cystic fibrosis (CF) HBECs while maintaining their capacity for differentiation (Peters‐Hall et al., 2018) Similarly, we were able to extend the life span of CF and non‐CF bile duct epithelial cells and maintain primary‐like cell characteristics, including multipotent differentiation potential and CFTR expression. This evidence concerns the gene CFTR and colorectal carcinoma.