Immunoblot analysis revealed that both P1 and P2 increased PC1 protein expression across all three patient-derived ADPKD cell lines (PKD1 Q2556X, PKD1 Q2641*, and PKD1 pR3926Afs*34) as well as in the Pkd1RC/– mouse line (Fig. 6A). The gene discussed is PKD1; the disease is autosomal dominant polycystic kidney disease.