Western blot confirmed increased cytosolic SLPI in transfected clones compared with parental and Mock controls: 1.00 (MMNK-1), 1.33 ± 0.39 (Mock), 3.22 ± 0.89 [SLPI(1)], and 5.03 ± 0.29 [SLPI(2)], while endogenous levels were higher in CCA cells (6.87 ± 0.17 in KKU-213A and 8.87 ± 1.23 in KKU-213AL5) (Fig 3a,b). This evidence concerns the gene SLPI and cholangiocarcinoma.