Interestingly, the expression profile of the predicted_AML cells suggests transformation towards malignancy, including changes in immune modulation (downregulation of the immune inhibitory molecules CTLA4 and LAIR1), and changes in molecules associated to tumour progression (increased expression of CCNA1, HLA-C and WISP3) (S1 Fig and Fig 3A). Here, CTLA4 is linked to acute myeloid leukemia.