Mutations in ASPM account for nearly 50% of all reported primary microcephaly cases in the Pakistani population, including 94 nonsense/missense variants, 88 small deletions, 16 small insertions, 2 gross deletions, 18 splice-site changes, and 1 complex rearrangement, totalling 218 pathogenic variants (HGMD Professional 2025.1). Here, ASPM is linked to Primary microcephaly.