Ca(OH)2 also targets the co-activator p300.60 Similarly, in rat ROS 17/2.8 cells and BMSCs, Ca(OH)2 (0.4 mmol/L, 6 and 12 h) stimulates BSP mRNA and protein expression via PKC, PKA, PI3K and ERK1/2 signalling pathways and CRE1/2 and FRE promoter elements.61 Matrix metalloproteinase 9 (MMP-9) (400 ng/mL) rapidly induces BSP protein in human breast adenocarcinoma (BAC) MDA-MB-231 cells (25 min),62 while MMP-2-deficiency increases BSP mRNA in murine BMSCs,63 suggesting a suppressive function. Here, MMP9 is linked to minimally invasive lung adenocarcinoma.