For this purpose,within the scope of this study, it is possible to analyze variationsin biological activity associated with specific derivatives, as eachacetophenone unit possesses a different aromatic substitution property.Furthermore, MCF-7 breast cancer cells, which are estrogen receptor-positive(ER+) and represent a well-characterized luminal A subtypeof breast cancer, were used to evaluate the cytotoxic and apoptosis-inducingpotential of the newly synthesized compounds as candidates againstchemotherapeutic agents with similar mechanisms. Here, ESR1 is linked to breast cancer.