NOS3 and endothelial dysfunction: Chronic experiments dispelled the notion of habituation: up to 28 days of exposure produced sustained endothelial dysfunction, hypertension, and inflammation with progressive vascular/cerebral ROS; whole-blood oxidative bursts peaked at days 4-7, Nos3 and FOXO3 declined in brain, and Vcam1 rose across groups (Frenis et al., 2021[30]).