Through ALNM-stratified single-cell RNA sequencing profiling, we identified 3 hallmark immune subsets in ALNM+ PBC: (a) proliferative MKI67+ T cells, (b) exhausted GZMA+ CD8+ T cells, and (c) CCL13/CXCL10/TOP2A+ macrophages. This evidence concerns the gene CD8A and primary biliary cholangitis.