IL7R and rheumatoid arthritis: Methods and results: CD4+CD25+CD127- Tregs were isolated from the peripheral blood of healthy donors and RA patients, then expanded in TexMACS medium supplemented with rapamycin (100 nM), IL-2 (1,000 IU/mL), gentamycin (50 μL/mL), and anti-CD3/CD28 Dynabeads.