Moreover, survival analysis performed through the Kaplan–Meier Plotter platform (https://kmplot.com) in a rectal adenocarcinoma-specific dataset further confirmed that elevated GNB2 expression predicted worse outcomes, with a hazard ratio of 3.01 (95 % CI, 1.31–6.91; log-rank p = 0.0067; Fig. 6D). This evidence concerns the gene GNB2 and rectum adenocarcinoma.