AXIN1 and hepatocellular carcinoma: To test whether alternative translation initiation is physiologically relevant in the context of liver cancer, we evaluated AXIN1 expression in the HCC cell lines JHH5 and JHH6 that both harbor 5′ truncating mutations in exon 2 (Q6Rfs∗22 and E5Dfs∗2, respectively) (Figure 2H).