To determine the effect of mTORC1 activation within osteoblasts on AML progression in vivo, we performed a second transplantation in which BM mononuclear cells (BM-MNCs) from WT-AML mice were transferred into lethally irradiated control and Col1a1-Cre;Tsc1fl/fl recipient mice (Figure 2B). The gene discussed is COL1A1; the disease is acute myeloid leukemia.