We drew the conclusion that quercetin inhibited the invasion and migration of LOVO cells through suppressing the ROS and HIF-1α expression and downregulating PI3K/AKT pathway stimulation, also inhibited the proliferation of both LOVO cells and HT-29 ​cells by inducing autophagy and apoptosis under hypoxia, and increased the anti-colon cancer effects of 5-FU. The gene discussed is AKT1; the disease is malignant colon neoplasm.