To further investigate the mechanism underlying the gain-of-function effects of WNT5B on striatal astrocytes in HD, we delivered an AAV-GFAP-WNT5B-P2A-mCherry virus into the dorsal striatum of N171-82Q mice (N171-82Q + WNT5B) and performed RNA-seq on striatal tissue extracts (Fig. 2a). This evidence concerns the gene WNT5B and Huntington disease.