WNT5B signals through both the canonical (β-catenin-dependent) and noncanonical (β-catenin-independent) WNT pathways.23 To determine which pathway predominates under HD-related pathological conditions, we overexpressed WNT5B in AAV-mHTT (103Q)-transduced human astrocytes and analyzed the expression of CTNNB1/β-catenin, MMP14, and NFAT family genes via qPCR (Fig. 3e). Here, WNT5B is linked to Huntington disease.