We validated its suitability by confirming the presence of mHTT aggregates within S100β-positive astrocytes, which is consistent with prior reports of noncell autonomous, prion-like propagation of mHTT from neurons to glial cells.32,33 These findings substantiate the N171-82Q model as a valid experimental system for dissecting astrocyte-mediated mechanisms of pathology in HD. The gene discussed is S100B; the disease is Huntington disease.