To further examine the role of TDP-43 in regulating endogenous GSK3 activity, we performed reciprocal siRNA-mediated knockdown of TARDBP in SH-SY5Y neuroblastoma cells, achieving a mean knockdown of 75% TDP-43 protein by 72 h (Supplementary Fig. 1a–c). The gene discussed is TARDBP; the disease is neuroblastoma.