ONECUT2 and metastatic malignant neoplasm in the lymph nodes: ONECUT2 DNA methylation was statistically significantly associated with indicators of disease aggressiveness, such as high Gleason grade (p = 0.0101 and AUC = 0.68) (3, 4, 5), T3, extraprostatic extension (EPE) positivity (p = 0.0423 and AUC = 0.65), particularly for seminal vesicle (SV) invasion positivity (p = 0.0024 and AUC = 0.76), and positive lymph node metastasis (p = 0.0005 and AUC = 0.80) (Fig. 5B-F).