Heatmap representation of unsupervised clustering analysis revealed distinct DNA methylation patterns in adjacent-normal prostate and PCa lesions with statistically significant cancer-specific DNA hypermethylation (p < 1.9E-16 and 1.2E-15, respectively) in 7–8 CpGs in the ONECUT2 locus (Fig. 4B). Here, ONECUT2 is linked to cancer.